Anti-spasmodic and Gastroprotective Activities of Harungana madagascariensis Leaf: A Traditional Anti-diarrhoea Remedy

Pharmacognosy Research,2022,14,4,492-498.DOI:10.5530/pres.14.4.71Published:October 2022Type:Original ArticleAuthors:Adaobi C. Ezike, Nwoyi N. Bassey, Emenike Amah, Daniel U. Nwankpa, Aniebiet E. Samuel, and John O. Medewase Author(s) affiliations:Adaobi Ezike*, Department of Pharmacology Toxicology, Faculty Pharmaceutical Sciences, University Nigeria, Nsukka, Enugu, NIGERIA. Abstract:Background: Harungana madagascariensis Lam. ex Poir. (Hypericaceae) leaf extract is used by some communities in the Niger Delta to treat diarrhoea, ulcers, wounds. Objectives: This study investigated antispasmodic, antimotility gastroprotective properties methanol:dichloromethane (1:1) H. leaves (HME). Materials Methods: The antispasmodic activity was evaluated vitro using actions on contractions guinea pig ileum rabbit jejunum provoked spasmogens. HME gastrointestinal transit assessed vivo normal defaecation charcoal meal time tests rodents. gastric ulcers produced ethanol indomethacin were investigated. also subjected phytochemical analysis acute toxicity tests. Results: suppressed isolated elicited histamine acetylcholine. significant (P<0.05) reduction (12.50 - 100%) propulsion mice (17.60 43.08%). Additionally, significantly prevented both ethanol- indomethacin-induced stomach ulcers. An oral LD50 >5000 mg/kg obtained an assay HME. Conclusion: findings showed that has gastroprotective, properties. Keywords:Charcoal test, Gastric lesions, Gastrointestinal motility, Guinea ileum, Rabbit jejunum, Tyrode solutionView:PDF (875.22 KB)